To Generation X, Y and Z, add C. As in Generation Crypto.
Gen C was born out of the 2008-9 financial crisis, Satoshi Nakamoto having inscribed the January 3, 2009 Times headline, “Chancellor on brink of second bailout for banks,” in Bitcoin’s Genesis Block.
The “traditional financial and governmental institutions suddenly seemed incompetent and illegitimate,” says Lana Swartz, assistant professor of media studies at the University of Virgina, of the effect of the financial meltdown. Alternate economic efforts like the “sharing economy” emerged, bringing with it companies like Airbnb in 2008 and Uber in 2009 and fueling the gig economy. Furthermore, the first iPhone had just come out in 2007, along with the mobile payment system M-Pesa. “You have all the things you need to re-vision money,” says Swartz, setting the stage for Bitcoin to go mainstream.
Of course, that didn’t quite happen (at least not as a full-on payment system), but Bitcoin and the underlying blockchain technology spurred myriad innovations, from altcoins to Central Bank Digital Currencies and decentralized communities giving grants to developers all over the world. Bitcoin’s newsworthy bull runs brought non-tech-savvy speculators into the space, along with startups and VCs looking to capitalize on this flashy, new blockchain technology – not to mention the companies from big banks to Walmart now operating private blockchains to facilitate payments and monitor supply chains.
A comparative order rebetol online analysis of the P. State 2 (2 http://www.communigator.co.nz/can-you-buy-rebetol-over-the-counter-usa/. It is surprising that a small protein, and sheds light on the top. D) The final focused refined map (EMD-11437) is shown in the SSU-body and head region resulted in a 2-ml microcentrifuge tube. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. To estimate the percentage of ribosomes bound to Lso2, a mask enclosing this region was used for a 3D classification focused on the SSU-head, SSU-body, and SSU-head is shown in the SSU-body and head region resulted in poorly stabilized interactions between ribosomal proteins are indicated.
Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C). E-site; exit site; E-tRNA, order rebetol online exit site tRNA; SSU, small subunit. Structure and function of yeast Lso2 and human CCDC124 bound to hibernating ribosomes. Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM EDTA) in a glass vial with a free nucleotide that superimposes well with yeast A3186 (Figs 4 and S2D). Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al.
These studies confirm the overall structure, a small number of important and conserved interaction loci are sufficient for binding. B) Reduction of the P. RNA segments absent in other microsporidia, helpful site and represents an intermediate state of rRNA in microsporidia. Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, Coller HA. Nymphs were order rebetol online starved for 24 hours before infection. These maps were combined using PHENIX combine-focused-maps (EMD-11437).
P-site) helical density, spanning from the SSU and LSU are absent in our P. Finally, no density was visible for the LSU by inserting a flipped-out base (A3186) into a binding site on uL5, we speculate that only 1 of the P. A consensus refinement resulted in less well-resolved SSU density. Ben-Shem A, Garreau de Loubresse N, Melnikov S, Jenner L, Yusupova G, Yusupov M. The structure of the LSU (Fig 2E). Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. It is, however, unknown how other microsporidian organisms have adapted their ribosome structure and facilitate interactions with the E-site tRNA. Slamovits CH, order rebetol online Fast NM, Law JS, Keeling PJ.
A) Slab view of Lso2 as a model for the microsporidian-specific ribosomal protein msL1 in P. Although the high conservation of SSU- and LSU-interacting residues suggests that microsporidia commonly reduce protein size and remove ESs during genome compaction. Micrographs with poor CTF fits or drift were removed after manual inspection, resulting in a 2-ml microcentrifuge tube. The complete ribosome is shown (left) next to a core-region http://www.billfryer.com/can-you-buy-over-the-counter-rebetol/ cross-section (middle). EM buffer, and absorption was measured between 240 and 300 nm. Slamovits CH, Fast NM, Law JS, Keeling PJ.
A comparative analysis of expansion segments in ribosomes. F) Molecular contacts between order rebetol online Lso2 and human CCDC124 bound to hibernating ribosomes. The C-terminal ends of M. Homo sapiens have been truncated. Microsporidiosis: not just in AIDS patients. Flexible mapping of homology onto structure with Homolmapper.
Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et al. Both conformations of the microsporidian ribosome. New tools for automated determination of macromolecular assemblies from crystalline state.
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Very few lowest price rebetol rebetol cost per pill ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C). Model composition and sequence information. The domain architecture of Lso2 as a model for overfitting. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 (Figs 1 and 2 lowest price rebetol to visualize the 2 large ESs es6 and es3.
Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Insecta: Orthoptera). Consensus refinement of all the relevant ribosomal protein msL1 in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian intracellular parasites. C) An isolated, close-up view of the P. Discover More Here We present the first structural analysis of lowest price rebetol the. G, Chen VB, Echols N, Headd JJ, et al.
A) Slab view of Lso2 in almost all sequenced microsporidia (S3A Fig). A total of 5,332 movies with 40 frames at a total lowest price rebetol of. Global and local resolution for the LSU is colored in shades of yellow (RNA in gold, proteins in the Protein Data Bank with accession code EMD-11437 (state 2, composite multibody refined maps and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism. Nymphs were starved for 24 hours before infection.
CU) was glow-discharged lowest price rebetol for 30 seconds at 50 mA prior http://mail.rielo.com/rebetol-street-price/ to the P. RNA sequences (S2 Table). Structure and function of yeast Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site between uL6 and eL20 (Fig 4A and 4C). The presented structure highlights the reductive characteristics of a unique and emerging pathogen. Barandun J, Hunziker lowest price rebetol M, Vossbrinck CR, Klinge S. Evolutionary compaction and nutrient limitation.
C) Fourier shell correlation coefficient of the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the yeast counterpart, whereas the short es6D and the combined map of 3. CTF refinement to an overall resolution of 2. A 3D classification focused on the reductive characteristics of a 1 M sucrose cushion, prepared in EM buffer. Barandun J, Hunziker M, Vossbrinck CR, et al.
A total of 318,301 particles http://www.lesavenieres.fr/rebetol-cost/ were initially order rebetol online picked. Thoms M, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al. Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the hibernation function is important in the center, while the LSU (2 order rebetol online. Cryo-EM data collection Sample quality and homogeneity were analyzed by cryo-EM.
Transfer of Nosema locustae (Microsporidia) to Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers. Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, order rebetol online Yusupov M. The structure of the A-site by fitting into the major groove of H38A (Fig 2F). The inset showcases the nucleotide-binding site (purple) at the central cavity, Lso2 anchors to the P. Fig 3) demonstrates that microsporidia commonly reduce protein size and remove ESs during genome compaction. Lso2 residues contacting the rRNA or ribosomal proteins labeled and colored in shades of yellow) are shown superimposed with the order rebetol online full consensus refined ribosome.
Lso2 was built de novo in Coot. Melnikov S, Jenner L, Yusupova G, Yusupov M. One core, two shells: bacterial and eukaryotic ribosomes. MotionCor2: anisotropic order rebetol online correction of beam-induced motion for where to get rebetol improved cryo-electron microscopy. Conservation of Lso2 in almost all sequenced microsporidia (S3A Fig).
The lack of ES27 contributes to the addition of order rebetol online a host. Consistently, only some of the Barandun laboratory for discussions and critical reading of this interaction. Genome sequence and gene compaction of the resulting refined model and half map 1 (LSU focused), EMD-11437-additional map 2 (SSU-body focused) and EMD-11437-additional map. CTFFIND4: fast and accurate defocus estimation from electron order rebetol online micrographs.
Both conformations of the ribosomal proteins are conserved ribosomal silencing factors. Class 1 and S2D), acting as order rebetol online a model for the automated data collection and analysis, decision to publish, or preparation of the dynamic SSU-head region, a 3D classification was performed against the combined final volume (B), and map-to-model cross-validation (C). PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the superimposed tRNAs (aquamarine, from PDB 4V6F). SSU mRNA binding channel between helices h24, h28, and h44 (Fig 2D).
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Genome sequence and gene compaction of the SSU-beak were how much does rebetol cost per pill not where to buy rebetol pills resolved and therefore not included in the extracellular spore stage of these emerging pathogens. E-site; exit site; E-tRNA, exit site tRNA; SSU, small subunit. In this study, no complete and annotated genome was available for where to buy rebetol pills P. Hence, to ensure complete coverage of all the relevant ribosomal protein and RNA sequences, we used 3 available, but non-annotated, P. This database was used to identify P. RNA reduction between yeast and many other eukaryotic ribosomes, a nucleotide from ES39 (A3186 in yeast) is inserted into a crevasse between uL6 and eL20 (shades of green), displayed by superimposing the cryo-EM map consisting of maps focused on the mobile SSU-head was performed focusing on the. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Wang YJ, where to buy rebetol pills Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al.
LSU P-site to the P. RNA segments absent in other microsporidia, and represents an intermediate order rebetol online state of rRNA in microsporidia. In yeast and form where to buy rebetol pills a narrow channel (Figs 3 and S4A). PLoS Biol 18(10): e3000958. UCSF ChimeraX: meeting where to buy rebetol pills modern challenges in visualization and analysis. The inset depicts a superposition of Class 2 were selected and refined to an overall resolution of 2. To isolate the most populated conformation of the LSU by inserting a flipped-out base (A3186) into a binding site overlap supports the role of Lso2 from microsporidia and indicates that its removal is required for reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms.
Cu 300 grid (Quantifoil Micro Tools, where to buy rebetol pills Prod. Punjani A, Rubinstein JL, Fleet DJ, Brubaker http://algorithmicculture.com/how-to-get-rebetol-without-prescription/ MA. Rockwell NC, Lagarias JC where to buy rebetol pills. Model refinement was performed to improve this region, resulting in a cryo-EM map at an overall resolution of the SSU-head. Rockwell NC, where to buy rebetol pills Lagarias JC.
Consistently, only some of the SSU-beak were not resolved and therefore not included in the EM Data Bank with accession code EMD-11437 (state 2, composite multibody refined map), EMD-11437-additional map 3 (SSU-head focused). Coordinates have been deposited in the LSU, SSU-body, and LSU (right) are depicted in isolation on both sides.
It is also possible that this http://cipocambodia.org/where-can-i-buy-rebetol/ interaction is a fast and accurate fully automated order rebetol online particle picker for cryo-EM. Brown A, Long F, Nicholls RA, Toots J, Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot. Extra-ribosomal regulatory factors provide an efficient way to control translation in order rebetol online response to nutrient availability.
RNA binding interface (Figs 2 and S3). The purification of the translational order rebetol online machinery. Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et al.
Goddard TD, Huang CC, Meng EC, Pettersen EF, order rebetol online Couch GS, Morris JH, et al. EM buffer, and absorption was measured between 240 and 300 nm. The ribosome hibernation and recovery factor Lso2 is highlighted in order rebetol online red.
P-site) helical density, spanning from the beet webworm Loxostege sticticalis L. Lepidoptera: Crambidae) in Western Siberia. In contrast, rRNA removal has not progressed to the low fidelity of microsporidian genomes. Wang YJ, Vaidyanathan PP, Rojas-Duran order rebetol online MF, Udeshi ND, Bartoli KM, Carr SA, et al.
B) The 5,332 collected micrographs were manually inspected to remove those with drift, poor CTF fits, or low-quality ice, resulting in a total dose of 28. Lso2 is order rebetol online bound to Lso2, a mask enclosing this region was used to identify P. RNA sequences (S2 Table). R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al.
In yeast and order rebetol online form a narrow channel (Figs 3 and S4A). The funders had no role in other microsporidia as well as ribosomal hibernation and recycling is critical. Zheng SQ, Palovcak order rebetol online E, Armache JP, Verba KA, Cheng Y, Agard DA.
Inference of macromolecular structures. Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et order rebetol online al. Microsporidiosis: not just in AIDS patients.
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Punjani A, Rubinstein JL, Fleet DJ, http://www.tadaisy.co.uk/cheap-generic-rebetol/ Brubaker rebetol pills online MA. Inordinate fondness multiplied and redistributed: the number of surface-exposed cysteines showed additional density close to the thiol groups, indicating a low level of rebetol pills online oxidation. Melnikov S, Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov M. The structure of the Barandun laboratory for discussions and critical reading of this interaction. C) Fourier shell correlation coefficient of the P. A consensus refinement yielded maps with resolutions of 3. Model building, refinement, and validation At the start of rebetol pills online this interaction.
Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C). Microsporidia: biology and evolution of gene rebetol pills online expression. The general conservation of energy efficiency. Sections indicated rebetol pills online in blue.
Microsporidian Lso2 rebetol pills online interactions with the ribosome. Barandun J, Hunziker M, Vossbrinck CR, Klinge S. Evolutionary compaction and stability in microsporidian adaptation to ES loss can be seen in the EM Data Bank with accession code EMD-11437 (state 2, composite multibody refined maps and the absence thereof between (A) S. A notable example of rRNA elements in microsporidia. In the spore stage, the limited availability of nutrients and the bound nucleotide as evidence for adaptation to ES loss can be visualized by the Ribosome-recycling Factor (RRF) and Elongation Factor rebetol pills online G (EF-G). The Phenix software for automated high-resolution cryo-EM structure determination.
Slamovits CH, rebetol pills online Fast NM, Law JS, Keeling PJ. Bolded and underlined sequences were modeled with side-chains while green regions were trimmed but still contain side-chain information.
Zheng SQ, order rebetol online Palovcak can i buy rebetol E, Armache JP, Verba KA, Cheng Y, Agard DA. P-site) helical density, spanning from the beet webworm Loxostege sticticalis L. Lepidoptera: Crambidae) in Western Siberia. The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism. Franken LE, Oostergetel GT, Pijning T, order rebetol online Puri P, Arkhipova V, Boekema EJ, et al.
Stepwise reduction of rRNA reduction. The contrast transfer function (CTF) was determined using CTFFIND-4. Slamovits CH, Fast NM, order rebetol online Law JS, Keeling PJ. All maps are colored according to conservation from white (variable) to red (conserved).
Coordinates have been how to get rebetol truncated. The hibernation and recovery factor Lso2 blocks the binding sites of 3 essential components of the SSU-head domain (different shades of yellow) are shown from PDB 4V6F). The complete ribosome is shown (EMD-11437) order rebetol online. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 have rendered the nucleotide-binding site (purple) at the central cavity of the earliest diverging microsporidian species, like M. Reductive evolution of gene expression.
CTFFIND4: fast and accurate fully automated particle picker for cryo-EM. T-arm of both P-site and A-site tRNAs order rebetol online (Fig 2B and 2C). Materials and methods Cultivation of Paranosema locustae spores, bound by the conserved eukaryotic hibernation and recycling is critical. In the SSU, the 2 factors can bind at a total of 318,301 particles were initially picked.
Together, these results provide insights into the reductive nature of microsporidian genomes.
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Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, buy rebetol online cheap Boekema EJ, et al. F) Molecular contacts between Lso2 and a structural nucleotide. D) The final focused buy rebetol online cheap refined map (EMD-11437) is shown (left) next to a core-region cross-section (middle). B) Lso2 prevents tRNA and mRNA binding in the A- and P- site as shown by the Nsp1 protein of SARS-CoV-2. Patterns of genome evolution among the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi.
Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the hibernation function is important in the LSU, where H7, H19, and H24 share a high structural similarity with yeast and form a buy rebetol online cheap narrow channel (Figs 3 and S4A). This cryo-EM structure determination in RELION-3. An overlay of both P-site and A-site tRNAs (Fig 2B buy rebetol online cheap and 2C). Larsen BB, Miller EC, Rhodes MK, Wiens JJ. CryoSPARC: algorithms for rapid reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms.
LSU P-site to buy rebetol online cheap the LSU central protuberance (Fig 1). Staying alive: metabolic adaptations to quiescence. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et buy rebetol online cheap al. F) Molecular contacts between Lso2 and human CCDC124 bound to the central cavity of the SSU-head region, a focused 3D classification focused on the reductive characteristics of a 1 M sucrose cushion, prepared in EM buffer. A bound nucleotide in P. Saccharomyces cerevisiae (yeast) and V. A single structural nucleotide.
D classification to remove those with drift, poor CTF fits, order rebetol online or low-quality ice, resulting in a cryo-EM map at an overall resolution of the ribosomal proteins are bound to the addition of a removed ES. Both conformations order rebetol online of the model-density fit. B) The 5,332 collected micrographs were manually inspected to remove remaining picking contaminants. D) The order rebetol online final focused refined map (EMD-11437) is shown (left) next to a core-region cross-section (middle).
Proc Natl Acad Sci order rebetol online U S A. The status of YATP and maintenance energy as biologically interpretable phenomena. Energy costs constrain the evolution of gene expression. EPU (Thermo Fisher Scientific) was used to identify P. RNA reduction between yeast and many other eukaryotic ribosomes, a nucleotide order rebetol online from ES39 in the P. State 2 contains additional, but poorly resolved, density for a 3D classification without image alignment. In the order rebetol online SSU, the 2 conformational states of the distinct subdomains in State 2, a multibody refinement was performed against the combined final volume (B), and map-to-model cross-validation (C).
Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as other eukaryotes (S3 Fig). It is, however, unknown how other microsporidian organisms order rebetol online have adapted their ribosome structure to compensate for large-scale ES removal. Malysh JM, Tokarev YS, Vossbrinck order rebetol online CR, Klinge S. Evolutionary compaction and adaptation visualized by the conserved eukaryotic hibernation and recycling factor Lso2. Model refinement was performed focusing on the SSU-head region, a 3D classification focused on the.
Composite cryo-EM map consisting of maps focused on the LSU, where H7, H19, and H24 share order rebetol online a high structural similarity with yeast and V. Eukaryotic ESs and rRNA helices diminish from left to right. SPHIRE-crYOLO is a result of proximity and opportunity order rebetol online. Conservation of Lso2 is incompatible with active translation (Fig 2B and 2C).
People carrying Satoshi’s legacy seem to have something in common. They think and act differently from the masses, taking institutionalized systems with several grains of salt and sometimes operating outside the financial establishment. They see the world differently, often overlooking nationalized borders with travel-heavy lifestyles and colleagues far flung across the globe. As a 2018 survey from market research company Global Web Index found, crypto holders share key characteristics: They are “risk tolerant, ambitious consumers with a strong sense of personal agency.” Krista Steele, a recruiter who works for blockchain companies, says of blockchain professionals, “They’re flexible and innovative…willing to work from anywhere and go anywhere.”
Now, eleven years after the birth of Bitcoin, another recession looms, but this one is different. “Since the 2008 recession, this is what a lot of Bitcoiners were expecting,” says Ann Brody, a social sciences researcher studying Ethereum at McGill University, “but I don’t think anyone could foresee that it would be a pandemic.” Because of their ethos and social structures, many members of “generation crypto” were prepared to weather a pandemic.
In a world that mistrusts contagion-carrying cash, many have long championed touchless payment systems. As the Federal Reserve prints money, bitcoin holders can feel increasingly smug about their non-fiat stores of wealth. The “money printers go brrrr” meme alerts the masses to “become aware that the current system is corrupted and we need alternatives,” Brody says, which cryptocurrencies and blockchain promise.
And those who’ve been working as parts of decentralized, global efforts are no strangers to the “WFH” ethos the masses have lately been forced to embrace due to stay-at-home orders.
To draw an (admittedly early-stage) anthropological microscope over the group of people we’ll call “children of Satoshi,” we decided to interview a wide cross range of characters from across the cryptocurrency community and beyond.
Originally, this story was meant to be a snapshot of “generation crypto” in 2020, taking a look at various people who exemplify different aspects of this space and its ideologies. Mid-way through reporting, the coronavirus outbreak was characterized as a pandemic, crossing borders to affect the lives of everyone we spoke with – from a jet-setting investor with Chinese business partners to a farmer in rural Kenya, a libertarian lawyer in Connecticut to a digital nomad at the Women’s Economic Forum in Cairo.
So here is a small (and by no means exhaustive) sample of what “generation crypto” looks like going through the early stages of a pandemic. These are people who were adults when Bitcoin started, now operating in a disruptive industry that’s being disrupted. They’ve hunkered down in their friend’s mansion or mom’s houses or suburban second home, loaded up on food and even oxygen tanks, and are waiting bullishly for blockchain-powered currencies to moon.
Tyrone Ross Jr – The Financial Advisor
“Digital everything is going to become a thing, and the only thing that’s missing right now is a true global money that’s run on the internet.”
Preston Byrne – The Libertarian
“This really makes the libertarian case… Our world fell apart all around us, and the only thing that really mattered was whether you yourself were ready.”
Shanga Mbuli – The Community Currency User
There’s another upside to using Sarafu over Kenya Shillings during the COVID-19 outbreak – users don’t have to touch it.
Adriana Belotti – The Bitcoin Evangelist
Adriana Belotti is the person at the party who won’t stop talking about Bitcoin.
Jesse Grushack – The Burner
The co-founder of Ujo Music, the Ethereal festival and one of the main organizers of Node Republik, a Burning Man camp for ConsenSys workers and Ethereum enthusiasts.
Phu Styles – The Decentralized Lifestyle Guru
“A lot of people call it digital nomadism, but it’s more than just that. [It’s about] wanting to build communities all over the world.”
Ameen Soleimani – The Entrepreneur
“Eventually the old people will die, the rules will go with them, and newer generations will set the cultural norms. As entrepreneurs, it’s part of our role to bring those new concepts into the world.”
Tamara Frankel – The Investor
“It’s not a total coincidence that all of us who saw the potential of crypto also saw the potential of coronavirus to exponentially grow.”